Consultation and Training
If you have any questions regarding the services of the Cellular/Molecular Core Facility, contact Sheryl White
Cellular and Molecular Biology Core - DNA Services
- Construct design
- Vector modification
- Cloning and subcloning
- Bacterial transformation & colony screening
- Plasmid DNA preparation
- Probe construction and labeling
- Library construction and screening
- Filter array hybridization
- Site-directed mutagenesis
- Polymerase chain reaction (PCR)
- Promoter Analysis
Cellular and Molecular Biology Core - RNA Services
- Preparation of RNA from cells or tissue
- RNA amplification
- Reverse transcription (RT) to produce cDNA
- Quantitative PCR/RT-PCR
- RT-PCR and gel analysis of products
- Northern blot analysis
- Rnase protection assays
- Differential display analysis
- siRNA design
- in situ RNA analysis
Cellular and Molecular Biology Core - Protein Services
- 1 & 2D SDS-polyacrylamide gels
- Western blot analysis
- Nuclear, cytoplasmic and membrane protein extracts
- Electrophoresis mobility shift ("gel shift") assay
Cellular and Molecular Biology Core - Cell Culture, Histology and Microscopy Services
- Zeiss PALM Microlaser dissection of samples
- Zeiss PALM Microlaser system for sections and live cells
- Neuroleucida Morphometrics and Microscopy
- Culture of cell lines and primary cell cultures
- Transient and stable transfections
- Reporter assays: luciferase, beta-galactosidase, secreted alkaline phosphatase
- Sectioning: paraffin or frozen
- Histological stains: hematoxylin and eosin, cresyl violet
- Immunohistochemical procedures: immunofluorescence, peroxidase
- Leica Vibrotome
- Invitrogen Countess Cell Counter