The Harry Hood Bassett Flow Cytometry and Small Particles Detection (FCSPD) Facility at UVM is a Larner College of Medicine Core Facility designed as a multi-user resource for the high-speed analysis and sorting of cells. We are located on 1st floor of Firestone Medical Research Building (FMRB), in Firestone RM 161, part of the Center for Biomedical Shared Resources. The FCSPD Facility has BD LSRII and MACSQuant VYB analytical cytometers, and a BD FACS AriaIII high-speed cell sorter. This equipment was purchased through National Institute of Health and UVM-College of Medicine Shared Instrumentation Grants. Investigators who bring samples to the FCSPD Facility for sorting or analysis pay a fee, which is used to offset operating expenses. The FCSPD Facility provides professional consultation and assistance with equipment use, experimental design, and interpretation of flow data.


MACSQuant VYBInstrumentation

The FCSPD Facility has BD LSRII and MACSQuant VYB analytical cytometers, and a BD FACS AriaIII high-speed cell sorter.

Calculator ImageRates and Appointments

All fees are based on the amount of time scheduled. Analyzers have a minimum charge of 15 (VYB) and 30 (LSRII) minutes, and cell sorting has a minimum charge of 30 minutes. 

Test Tubes with black backgroundFlow Cytometry Protocols

In this section you will find the most common Flow Cytometry Protocols that investigators at the UVM-FCSPD Facility use.